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NOAnalysis manages the data from liquid, REB, bag and breath analysis.

NOAnalysis is a suite of four programs (Liquid, REB, Bag and Breath) for collection and processing of data from the NOA. Data is collected via the computer serial port and the programs can be run on a PC (Win 95/98/NT/XP/Vista) or a Macintosh PowerPC (System 7 or higher). All files are saved in a tab-delimited text format for easy import into a spreadsheet or statistics program.

Liquid

The Liquid program is used with the purge vessel to acquire data, name the samples, integrate the peaks (using threshold and peak width detection and automatic select of start and stop integration marks), prepare calibration curves, calculate the concentration of the samples and save the results. New features in Version 3.21 include:

• Create Sample Names Tables to identify samples prior to injection. Names can be added or deleted during data acquisition.
• Manual Integration option — skips peak detection and allows setting start and stop integration marks
• Change concentration units after calculation

Restricted Exhaled Breath (REB)

The REB program is for on-line measurement of exhaled NO. The program is used to acquire data, display feedback for the subject to insure constant flow exhalation, select NO plateaus using ATS criteria*, calculate the mean and average deviation from replicate measurements, and to reject outliers and save or print the results. New features in Version 3.21 include:

• Use of either pressure or flow feedback for constant flow exhalation
• New results printout including subject information, exhaled NO values, exhaled NO plots and optional image file for printing institution's logo
• New results file format, measurements from different days can be saved in a single results file

* Recommendations for Standardized Procedures for the Online and Offline Measurement of Exhaled Lower Respiratory Nitric Oxide and Nasal Nitric Oxide in Adults and Children-1999, Am J Respir Crit Care Med Vol 160. pp 2104?2117, 1999.

Bag

The Bag program is for the measurement of exhaled NO collected off-line in mylar bags. The program is used to acquire data, analyze NO levels in each bag, calculate averages for replicate samples from a subject, edit the number of samples per subject and save and print the results. During data acquisition, information about the sample can be entered into a table and NO levels set for each sample.

After data collection, outliers can be deleted and the results printed or saved in a tab-delimited text format for easy input into statistics or spreadsheet programs.

Breath

The Breath program is for breath-by-breath measurements of exhaled NO. Since this technique does not exclude nasal NO or provide for constant flow exhalations, it is only used for those subjects who cannot perform the on-line or off-line methods. The breath program is used to determine the time offset between the NO and pressure signals, acquire data, detect the beginning and end of each exhalation using the pressure signal, adjust the offset (if necessary) and calculate the maximum, minimum and end-tidal or plateau NO concentration for each breath along with overall mean and standard error. Outliers can be rejected and the results printed and saved.